EZ BioResearch Pre-poured Agar Plates and Swabs, 10 plates & 10 swabs Guide

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Features

• 10 Prepoured sterile LB-agar plates are ready to use. 10 sterile 6-inch long swabs (in 5 pouches).
• Laboratory quality LB-agar plates manufactured by EZ BioResearch LLC.
• Laboratory formulation of nutrient medium LB for guaranteed bacterial, yeast, and mold growth.
• Great for school science fair projects. No messy microwave or heating is needed. Safe for children of all ages.
• Free upgrade to Expediated Shipping Service.

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Product Description

What is inside the package:
1) 10 Pre-poured sterile LB-agar plates (100 x 15 mm Petri Dish)
2) 10 6-inch sterile swabs (in 5 pouches)

Storage condition and shelf life:
Store agar plates at 4 oC refrigerator with a shelf-life of up to 30 days.

Instructions:
Lysogeny broth (LB), a nutritionally rich medium, is primarily used for the growth of bacteria. LB media formulations have been an industry standard for the cultivation of Escherichia coli as far back as the 1950s. These media have been widely used in molecular microbiology applications for the preparation of plasmid DNA and recombinant proteins. It continues to be one of the most common media used for maintaining and cultivating laboratory recombinant strains of Escherichia coli (Wikipedia).LB-agar plates are made from nutrient medium (LB) and agar powder under sterile conditions. It contains a brown-yellowish nutrient agar layer in the bottom half of a Petri dish. This yellowish layer is used to culture bacteria and other micro-organisms. Sterile swabs are used to pick up bacteria and seed them on to the agar plates.
After the plates are swabbed, the plates should be incubated at 37 oC for optimal bacterial growth. Normally 24 hr incubation should be enough to see the appearance of bacterial colonies. Incubation at 25 oC or room temperature should take considerable longer time (2 to 3 days) to see bacterial colonies.

Notice:
1) The plates should be placed in an upside down position to prevent water condensation.
2) The plates should be placed in a closed box (NOT sealed) to minimize the chance of contamination.
3) The experiment should be terminated after 5 days. Prolonged incubation is strongly discouraged due to possible contamination in a non-sterile environment.
For more info, search internet using key words such as agar plates.

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